Functional Characteristics of Two Human MATE Transporters: Kinetics of Cimetidine Transport and Profiles of Iinhibition by Various Compounds

Authors

  • Kin-ya Ohta Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Katsuhisa Inoue Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Tomoya Yasujima Graduate School of Pharmaceutical Sciences, Nagoya City University
  • Munenori Ishimaru School of Pharmacy, Aichi Gakuin University
  • Hiroaki Yuasa Graduate School of Pharmaceutical Sciences, Nagoya City University

DOI:

https://doi.org/10.18433/J3R59X

Abstract

Purpose. Human multidrug and toxin extrusion protein 1 (hMATE1) and hMATE2-K are organic cation/H+ antiporters that have recently been identified and suggested to be involved in the renal brush border secretion of various organic cations. Information about functional characteristics of them has been accumulating, but still insufficient to fully understand their functions and respective roles. The present study was conducted to help clarify them. Methods. The cDNA of hMATE1 was isolated from human brain cDNA by RT-PCR and hMATE2-K cDNA was from human kidney cDNA. HEK293 cells were stably transfected with hMATE1 and hMATE2-K, and the cellular uptakes of [3H]cimetidine and [14C]tetraethylammonium (TEA) were evaluated. Results. It was first found that both hMATE1 and hMATE2-K can transport cimetidine with high affinities, indicated by small Michaelis constants of 8.00 mM and 18.18 mM, respectively. These were much smaller than those for TEA (366 mM and 375 mM, respectively, for hMATE1 and hMATE2-K). Subsequent investigation using cimetidine as a probe substrate into the profiles of inhibition of the two hMATEs by various compounds indicated that they are similar in principle but different to some extent in substrate recognition, reflecting the modest differences in amino acid sequences between them. In fact, cimetidine transport by hMATE1 was correlated to that by hMATE2-K, which is 65% similar to hMATE1, but not as good as to that by rat MATE1, which is 86% similar. Conclusions. Cimetidine was demonstrated to be a high affinity substrate of both hMATEs. Subsequent evaluation of the inhibition of hMATEs by various compounds indicated no major difference in function or role between hMATE1 and hMATE2-K.

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Published

2010-01-06

How to Cite

Ohta, K.- ya, Inoue, K., Yasujima, T., Ishimaru, M., & Yuasa, H. (2010). Functional Characteristics of Two Human MATE Transporters: Kinetics of Cimetidine Transport and Profiles of Iinhibition by Various Compounds. Journal of Pharmacy & Pharmaceutical Sciences, 12(3), 388–396. https://doi.org/10.18433/J3R59X

Issue

Section

Pharmaceutical Sciences; Review Articles