Bicarbonate supplementation as a preventive way in statins-induced muscle damage

Authors

  • Masaki Kobayashi
  • Toshiki Kagawa
  • Katsuya Narumi
  • Shirou Itagaki
  • Takeshi Hirano
  • Ken Iseki Hokkaido University

DOI:

https://doi.org/10.18433/J33018

Abstract

ABSTRACT – Purpose. The aim of this study was to evaluate the bicarbonate-induced improvement of statins, cerivastatin, simvastatin acid and lovastatin acid -induced apoptosis using rat myoblast cell line (L6) as a model of in vitro skeletal muscle and of cerivastatin-induced muscle damage in vivo study. Methods. Statin-induced reduction of cell viability and apoptosis was measured by 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay and caspase assay. In vivo, we evaluated plasma creatine phosphokinase (CPK) level in cerivastatin-treated rat. Results. Bicarbonate prevented cerivastatin-, simvastatin- acid and lovastatin acid -induced reduction of cell viability, morphological change and caspase activation in L6 cells. Moreover, in the in vivo study, bicarbonate prevented cerivastatin-induced increase in CPK concentrations. Conclusions. These results from in vitro and in vivo studies support that bicarbonate supplementation prevented statin-induced muscle damage.

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Author Biography

Ken Iseki, Hokkaido University

Ken Iseki, Ph. D., Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12-jo, Nishi-6-chome, Kita-ku, Sapporo, Japan. E-mail: ken-i@pharm.hokudai.ac.jp

Published

2008-02-20

How to Cite

Kobayashi, M., Kagawa, T., Narumi, K., Itagaki, S., Hirano, T., & Iseki, K. (2008). Bicarbonate supplementation as a preventive way in statins-induced muscle damage. Journal of Pharmacy & Pharmaceutical Sciences, 11(1), 1–8. https://doi.org/10.18433/J33018

Issue

Section

Pharmaceutical Sciences; Original Research Articles