In vitro Anti-Inflammatory Effects of Hyaluronic Acid in Ethanol-Induced Damage in Skin Cells

Authors

  • Manuela G Neuman Departments of Clinical Pharmacology & Toxicology, University of Toronto
  • Radu M Nanau Departments of Clinical Pharmacology & Toxicology, University of Toronto, In Vitro Drug Safety and Biotechnology
  • Loida Oruña State Center of Quality and Control of Drug, Havana
  • Gabriel Coto Placentary Histotherapy Center - CHP, Havana Cuba; Research Biomedical Department, CIDEM, Center of Pharmaceutical Chemistry, Latino-Americana Faculty of Medicine, Havana

DOI:

https://doi.org/10.18433/J3QS3J

Abstract

ABSTRACT - Ethyl alcohol (ethanol) is commonly applied in cosmetic and pharmaceutical preparations, as well as disinfectant for chronic wounds. Objective: The present study aimed to appraise physiological levels of ethanol-induced damage in skin cells in vitro and the possible repair by hyaluronic acid (HA). In addition, we aimed to establish cytokine-chemokine networks in the cellular media and the modulation of cytokines such as tumor necrosis factor-alpha (TNF-B), interferon-alpha (IFN-α), transforming growth factor-beta (TGF-B), interleukins (IL) such as IL1-B and IL-6, as well as matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP). Design and Methods: We treated human A431 epidermoid skin cells and mouse fibroblasts with ethanol at a concentration of 100 mM over 24 hours (h). A separate experiment looked at the effects of 2 consecutive treatments with 100 mM ethanol for 24 h each. HA obtained from umbilical cord excision was used at two concentration levels (2% and 4%) to determine its efficacy in the treatment. We measured cytotoxicity and cytokine networks in the media. Results: Treatment of cells with 100 mM ethanol increased cytotoxicity, as well as the release of pro-inflammatory cytokines into the culture medium. Conclusions: Ethanol may induce cytotoxicity in skin cells by enhancing the effects of pro-inflammatory cytokines. HA reduced the amount of pro-inflammatory cytokines released into the media both in human A431 epidermoid skin cells and in mouse fibroblasts. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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Author Biography

Manuela G Neuman, Departments of Clinical Pharmacology & Toxicology, University of Toronto

Departments of Clinical Pharmacology & Toxicology, University of Toronto, In Vitro Drug Safety & Biotechnology, MaRS, Toronto, ON, Canada, M5G 1L7;

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Published

2011-12-01

How to Cite

Neuman, M. G., Nanau, R. M., Oruña, L., & Coto, G. (2011). In vitro Anti-Inflammatory Effects of Hyaluronic Acid in Ethanol-Induced Damage in Skin Cells. Journal of Pharmacy & Pharmaceutical Sciences, 14(3), 425–437. https://doi.org/10.18433/J3QS3J

Issue

Section

Pharmaceutical Sciences; Review Articles